Reducing Non-Specific Binding in Surface Plasmon Resonance Experiments
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چکیده
Non-specific binding is an important experimental parameter to control when using SPR systems. Non-specific binding is the binding of analyte to non-target molecules on the sensor surface, as illustrated in Figure 1. The effect of non-specific interactions is a false positive contribution to the signal in a sensorgram. It is important for users to recognize non-specific binding and to implement strategies to reduce or eliminate its effects to get accurate kinetic data.
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